FANCD2 Knockout cell line (U-2 OS）

FANCD2 Knockout cell line (U-2 OS） Order

Catalog#

YKO-H1157

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

FANCD2 Knockout cell line (U-2 OS)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:5

Species

Human

Gene Name

FANCD2

Gene ID

2177

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% McCOY's 5A+10%FBS

Target region

Knockout region: Exon 5 (based on transcript FANCD2-212)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FANCD2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-2 OS cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FANCD2 gene knockout FANCD2 Knockout cell line (U-2 OS) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

FANCD2 Knockout cell line (U-2 OS）

Catalog#

YKO-H1157

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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FANCD2 Knockout cell line (U-2 OS）

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